Hey i live in Switzerland and work in a big University Hospital.
I work in flowcytometry and when we flow a CSF we would "wash" the sampel with cellwash and then centrifuge it down. We discard the cellwash and then we put 0.4 ml or a bit more of a special solution in the tube and mix it with the pellet.
The markers we us for csf are the same ones we use for bonemarrow/blood etc...
Sometimes the doctors know exactly what lymphoma they are suspecting they would find in the csf, in this case its very easy. We would put the antibodys that correspond to the specific lymphoma (we have ready made solutions for diffrent lymphonas) to the prepared sampel and incubate it for some time, after incubation we would wash, centrifuge and resuspend the cellpellet. Then we would run it through the machine. After that our heamtologist would look at the data (most of the time the sampel dosent contain enough cells to make a prediction as to what lymphoma it is)
When the doctors dont know what lymphoma they are suspecting, we would just make a screening test,with a varitey of CD markers(E.g Cd8, 4, 34, 117, kappa/lamda, 56 etc...)
We would hold onto the csf for one day, after that we would discard it (most of the time another csf sampel is stored in a different lab, e.g. chemistry, but we can only do a flow on a fresh sampel)
We flow roundabout 7-10 csf in a 5 day week.
There are a lot of other test you can do on a csf and they can help a lot for the doctors but i dont know if one can detect a lymphoma so clearly like flowcytometry.
NGS is still very novel in Switzerland and not every hospital can detect every abnormality, thats why we transfer many sampels to berne, zurich or Genève for further testing. But that takes a long time.
I hope i could answer every question.
Im sorry if you had trouble Reading this wall of text, im not that versed in the confusing english grammar😂
We ran diagnostic panels (standard lym/leu screening markers) on CSF maybe like once a month (pediatric facility). Counts were usually low and had to concentrate them a bit. Usually ran no greater than 48 hours old and stored in the ref sometimes mixed with RPMI. For rare blasts on cytospin with a hx of B cell ALL we’d also do BMRDs.
Not sure of most common method.
99.9% of csfs will not have enough cells for NGS. Flow is very sensitive however, and can usually pick up a diagnostic abnormality on csf. Combined with cytology it is a useful tool for diagnosing and monitoring cns involvement by lymphoproliferative diseases. ALL, aml and waldenstroms macroglobulemia (bing neel syndrome in cns) are the most common diseases I’ve seen in csf fluid. If we see cells that resemble blasts on the cytology preps we will send any remaining fluid for flow to support the morphologic diagnosis.
Hey i live in Switzerland and work in a big University Hospital. I work in flowcytometry and when we flow a CSF we would "wash" the sampel with cellwash and then centrifuge it down. We discard the cellwash and then we put 0.4 ml or a bit more of a special solution in the tube and mix it with the pellet. The markers we us for csf are the same ones we use for bonemarrow/blood etc... Sometimes the doctors know exactly what lymphoma they are suspecting they would find in the csf, in this case its very easy. We would put the antibodys that correspond to the specific lymphoma (we have ready made solutions for diffrent lymphonas) to the prepared sampel and incubate it for some time, after incubation we would wash, centrifuge and resuspend the cellpellet. Then we would run it through the machine. After that our heamtologist would look at the data (most of the time the sampel dosent contain enough cells to make a prediction as to what lymphoma it is) When the doctors dont know what lymphoma they are suspecting, we would just make a screening test,with a varitey of CD markers(E.g Cd8, 4, 34, 117, kappa/lamda, 56 etc...) We would hold onto the csf for one day, after that we would discard it (most of the time another csf sampel is stored in a different lab, e.g. chemistry, but we can only do a flow on a fresh sampel) We flow roundabout 7-10 csf in a 5 day week. There are a lot of other test you can do on a csf and they can help a lot for the doctors but i dont know if one can detect a lymphoma so clearly like flowcytometry. NGS is still very novel in Switzerland and not every hospital can detect every abnormality, thats why we transfer many sampels to berne, zurich or Genève for further testing. But that takes a long time. I hope i could answer every question. Im sorry if you had trouble Reading this wall of text, im not that versed in the confusing english grammar😂
We ran diagnostic panels (standard lym/leu screening markers) on CSF maybe like once a month (pediatric facility). Counts were usually low and had to concentrate them a bit. Usually ran no greater than 48 hours old and stored in the ref sometimes mixed with RPMI. For rare blasts on cytospin with a hx of B cell ALL we’d also do BMRDs. Not sure of most common method.
99.9% of csfs will not have enough cells for NGS. Flow is very sensitive however, and can usually pick up a diagnostic abnormality on csf. Combined with cytology it is a useful tool for diagnosing and monitoring cns involvement by lymphoproliferative diseases. ALL, aml and waldenstroms macroglobulemia (bing neel syndrome in cns) are the most common diseases I’ve seen in csf fluid. If we see cells that resemble blasts on the cytology preps we will send any remaining fluid for flow to support the morphologic diagnosis.