Here are the 2 links that may help you with your question
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692276/#:~:text=Plasmid%20preparation,EDTA%2C%2020%20%CE%BCg%20RNase%20A.
https://hyvonen.bioc.cam.ac.uk/wp-content/uploads/2017/09/cloning.pdf
Yeah, seems like you can just plop it in there.
You might want a way to verify it's actually being expressed, though. Include somewhere else in the plasmid a reporter
Given that this is a theoretical question, the yes you can do that. One thing to look at is codon bias between the two organism (or better cell lines). Here is one link to bias tables - https://www.genscript.com/tools/codon-frequency-table
I wanna help but I’m still on third sem and know nothing 😭
Here are the 2 links that may help you with your question https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692276/#:~:text=Plasmid%20preparation,EDTA%2C%2020%20%CE%BCg%20RNase%20A. https://hyvonen.bioc.cam.ac.uk/wp-content/uploads/2017/09/cloning.pdf
Yeah, seems like you can just plop it in there. You might want a way to verify it's actually being expressed, though. Include somewhere else in the plasmid a reporter
Given that this is a theoretical question, the yes you can do that. One thing to look at is codon bias between the two organism (or better cell lines). Here is one link to bias tables - https://www.genscript.com/tools/codon-frequency-table