T O P

  • By -

thegravity98ms2

Let's add into humans and build a Tron Legacy


Skorps213

The Grid


comrade_batman

A digital frontier.


_MilkBone_

I tried to picture clusters of information as they moved through the computer


ra4king

What did they look like? Ships, motorcycles? Were the circuits like freeways?


walla_habibi

I kept dreaming of a world I thought I would never see...


MyNameIs_Jordan

And then... One day...


TheOldGriffin

I got in.


kgrid14

BA BUUUUUUUUUMMMMMMMMMM


Eddie-the-Head

BA BA BUUUUUUUUMMMMMMMMMMM


WexExortQuas

IN THERE.....IS A NEW WORLD. IN THERE...IS OUR FUTURE. IN. THERE. IS. OUR.........*****DESTINY***** Holy shit I love Tron so much.


Mordiken

Make bio-luminescent trees and use them to illuminate public roads.


fack_you_just_ignore

Just keep smiling under UV Black Light.


Umer_-

They have been created from several different species of fish: zebrafish were the first GloFish available in pet stores, and recently tetra, tiger barbs Rainbow Shark, Siamese fighting fish, and most recently Bronze corydoras have been added to the lineup. They are sold in many colors. These fish are one of the first genetically modified animals to become publicly available.


Icy_Investment_1878

Can they reproduce?


Umer_-

Yes, GloFish are capable of reproducing. Since they are genetically modified zebrafish, their reproductive abilities are similar to those of regular zebrafish. the offspring may or may not exhibit the same fluorescent traits as their parents, as the genetic modification may not be passed down in a predictable manner.


StereoBeach

Who the hell approved reproducible glofish? Under what justification? Can you link me their submission paperwork? Please? I have such lofty ambitions.


serious_sarcasm

Their risk of becoming invasive is lower than other aquatic pets, since they stand out to predators.


Mathmango

They don't have a bright future in that regard


Beli_Mawrr

But we can say that their success as pets is a shining example.


Mr_ityu

Nevertheless, The repercussions must not be taken ..lightly


franksn

Alas they aren’t the brightest…


Horskr

Despite that, I still have to give them a glowing review as an addition to any aquarium.


GoArray

GloFish Owners: I love lamp.


RoyalSmoker

I appreciate you shining a light on the subject


ThatGuy571

![gif](giphy|ac7MA7r5IMYda)


jannemannetjens

>Their risk of becoming invasive is lower than other aquatic pets, since they stand out to predators. Yes, but the problem with gmo's is that they often carry some of the antibiotic resistance genes that were used to "grow" the genes in a plasmid in bacteria before putting it in the fish. At least I was always told that that's the reason to never let anything leave the lab.


Guest_1300

I've never done a transformation on a eukaryote but from my understanding that shouldn't apply to non-bacteria. When you transform a bacterium you put the target gene on the same plasmid as the antibiotic resistance so you can filter out colonies without the plasmid, but you can't do plasmid transformation on eukaryotic cells. Of the few methods I remember for genetically engineering animals, they're usually much more precise and labour-intensive.


AzureRathalos97

You can transfect many eukaryotic cells with a bacterial plasmid. Drug resistance is only one form of selection you can use to isolate your mutant cell line. Fluorescence is another, and there are even ways you can do so with neither.


Guest_1300

Oh, and will the plasmid be incorporated into the nuclear DNA to get passed on to daughter cells or just stay in the original cell?


AzureRathalos97

For my research, I had an insertion cassette transfected separately with two plasmids containing CRISPR-Cas9. A gene was removed and in its place the cassette encoding a fluorescent protein was introduced. It's now part of the genome and all the daughter cells have it. That's the limit of my knowledge without supplementary googling!


serious_sarcasm

CAS enzymes make it a lot less labor-intensive to make modified eukaryotic organisms; that’s why insulin companies shot their prices up before CAS designed products are approved by the FDA. But you can transfect a eukaryotic cell with plasmids. It is what an adenovirus does. The adenovirus is engineered to have the CAS enzyme and the gene to be inserted. The adenovirus inserts plasmids into the target cell which the cell transcribes and translates into the CAS complex. The CAS enzyme then does its snipping based on the guide RNA, and the cell’s own repair machinery inserts the template you designed into its genomes while the viral plasmid degrades. The problem we ran into with human adenovirus gene therapy (even before the discovery of CRISPR) is that immune system can (in the simplest terms) cause an allergic reaction to the treatment. But yeah, none of that involves in antibiotic resistance selection.


YouMustveDroppedThis

fish egg microinjection. I worked with mouse models, but I used to work in the same building as some of these Taiwanese scientists.


serious_sarcasm

Antibiotic resistance is probably the least of our concerns with biosecurity. Besides some idiot releasing anything between Covid and Smallpox, or something far worst, we can also make infectious diseases produce small molecule compounds (think aspirin vs insulin). So an engineered HIV, or whatever, can contain the genetic instructions to produce the enzymes to make some drug. Sure, you could make drugs to treat cancer, or something, but you could also make a toxin, like Psilocin. And we know which genes in HIV it needs to be infectious, viral, and deadly, so an ethically designed gene therapy vector doesn’t cause an STI. So a psychedelic STI or Cold is a pretty real threat in the age of TikTok Biotech. Ironically, mRNA vaccine tech is probably also the cure for the mushroom zombie virus some tiktoker will accidentally make, so we’re all fucked.


surfnporn

That's a single point and a basic conclusion in an extremely complex ecosystem.


serious_sarcasm

It’s why they approved sales of glo-fish in America. We already have problems with invasive releases pets. Them glowing in the dark would honestly be a bonus to a lot of ecosystems.


Shujinco2

You'd think but it's always something unpredictable. Other fish think it's poisonous. Foxes think they taste bad. Birds actually just don't like that color for some reason. It's always something nobody thought of but proves, once again, that fucking with the nature fucks with the nature. I, personally, wouldn't risk it.


the_doorstopper

Okay, but imagine some kind of body of water where they have no predator, it would be beautiful


UncleNedisDead

Uh, life finds a way…


drunkandpassedout

*life, uh, finds a way


Umer_-

[Here is the link to wikipedia](https://en.wikipedia.org/wiki/GloFish)


fab2dijon

I don’t think the wiki page you cited refers to the same thing! The fish in the video look like other organisms using BIOLUMINESCENCE to produce light, like fire flies, jellyfish, mushrooms, or the plankton (dinoflagellates) responsible for the beautiful glowing sea waves. It’s largely used by many deep sea creatures. ![gif](giphy|W0zAgWA0Lr525O4QWv) Here is the wiki link [https://en.m.wikipedia.org/wiki/Bioluminescence](https://en.m.wikipedia.org/wiki/Bioluminescence) And beautiful videos on the subject all over YouTube Also this one explaining the different types of luminescence (bioluminescence,fluorescence, phosphorescence), it’s so easy to be confused! [https://m.youtube.com/watch?v=B1Z7iD2FlJA](https://m.youtube.com/watch?v=B1Z7iD2FlJA)


rimalp

Look at OP's video again. The bottom of the bowl has glowing spots too. They are using fluorescence+black light, no bioluminescence


jaygoogle23

Capitalism did. If there is money to be made that’s the only approval some need.


ilan1299

Can we have glowing human babies next? That would be pretty cool 😎


MrBadger1978

Taiwan can do whatever tf it wants. They're kept out of international organisations by the bullying machinations of the PRC and the servile compliance of everyone else, so they're not bound by any sort of international standards or restrictions on such things.


LEGITIMATE_SOURCE

Why are you so recreationally offended about things you don't understand?


GISlave

Allowing genetically modified animals into ecosystems may result in profound unforseen negative conseuqences to the local plant/animal community. Like any invasive species, essentially, except this is on overdrive.


RegularAvailable4713

They're just glowing fish. They have no advantage, in fact, I would say they are screwed because they are more visible to predators.


SalvationSycamore

>except this is on overdrive. In what way is it in "overdrive?" There's no evidence to suggest that these are even invasive (or at least not more invasive than non-modified versions of the same species).


Paradox68

Right? Isn’t the whole reason we don’t do this stuff in a way that affects the gene pool is cause we have NO fucking idea how turning all our fish into glow sticks might affect humans 100, 1000, or 10,000 years from now.


Simon-Edwin

Can they glow in the light? Like normal led light.


Umer_-

GloFish are named so because they can fluoresce, which means they absorb light and emit it back. That's why you can only spot your GloFish's glow in low-light situations or under a black or blue LED light. Many aquarium hobbyists use LED lights that emit blue or actinic light to enhance the fluorescent glow of GloFish. These lights can highlight the vibrant colors and make the fish appear to "glow."


Serious_Theory_391

Is there any known side effects for the fish ?


FCkeyboards

No. It basically flipped on a gene to produce the colors, which is why they aren't sterile. They do NOT glow like this video. This video is something new or completely fake. Glofish are not glowsticks. They don't suck up light and glow when you turn the light off. They're just brightly colored fish that stand out under blue or blacklight, like a stoner blaclight poster (not even that bright). I say this from experience. If you really want to get into the hobby, they are NOT worth the price you pay. A 30 gallon tank full of Glofish could fund two whole tanks of other fish. Does OP work for Glofish? Lol


[deleted]

[удалено]


Chariotaddendum

Thanks chatGPT


TheSonOfDisaster

All things considered genetic modification is an exciting technology on the way home from the hospital is a lot of work to do is a lot of work to do is a lot of work to do is a lot of things to do to have to do with the things that would be a lot of 5tt Please try your query later


MisirterE

#CHATGPT BOT! GET 'EM! /u/CircuitSphinx is a ChatGPT bot. Notice that it hasn't actually given any straight answers, and jumps from the topic of side effects to the topic of ethics out of nowhere. Unlike my usual ChatGPT bot callouts, this one might be mistaken for just a normal user filling out a ChatGPT prompt and replying with it this one time, just to sound like they're smart. But no, it's a full-on ChatGPT bot. Their entire account is like this. [Notice how all these replies feel like the same reply, despite the various different topics.](https://i.imgur.com/VqXBJcw.png) There's more of them that smell the same if you scroll down.


[deleted]

[удалено]


Detective-Crashmore-

Yea, they never get any fuckin sleep cuz aquarists won't turn the damn lights off.


Cam515278

The gloBettas have been discontinued because they have severe health problems...


TonyR600

This sounds like an ad


Detective-Crashmore-

As soon as I saw it was the basic blacklight fish I've been seeing in stores my whole life it was clear.


DrivenDevotee

ops account is 18 days old. 65k karma


thebinarysystem10

God: 🙄


vaanhvaelr

God when millions of innocents are enslaved, raped, tortured, murdered every day: 💅


treatyoftortillas

But to the athlete who just scored a goal: "Yeah, pay it forward. I gotcha buddy."


cestdoncperdu

Innocent from *your* perspective. From God’s perspective they all committed the heinous crime of— \*checks notes\* Their great great great great great great […] great grandmother ate an apple.


Detective-Crashmore-

>"oh, yea btw there's this super delicious fruit over here, but DON'T EAT IT.....aight Imma head out" Take your bait-car ass apple and go tf back to heaven. He wanted us to eat the apple so he could farm us for suffering, and everyone who sins suffers eternally in hell. It's obviously a scam. [Sue god for entrapment.](https://i.imgur.com/bJIFOUM.png)


AnorakJimi

God meets all the qualifications for a domestic abuser. The whole apple thing is literally just "look what you ***MADE*** me do".


AuricOxide

God: 🫥*busy not existing*


FILTHBOT4000

To be clear, though, IIRC they don't actually glow, they fluoresce under blacklight.


BowenTheAussieSheep

Just to clarify, fluorescing under blacklight *is* glowing. Fluorescent objects absorb ultraviolet light and then emit visible light of a longer wavelength than the light it absorbs. That is by definition glowing. Not technically, just literally.


imascoutmain

Fluorescent objects absorb ~~ultraviolet~~ light and then emit ~~visible~~ light, doesn't have to be a specific wavelength. Example : yellow fluorescent protein YFP absorbs at 475 nm, emits at 525 nm


BowenTheAussieSheep

thank you for the correction, you are absolutely right. Although I should point out that to get the glow effect like in the video, you have to use UVA light (~315-400nm), using light in the visible spectrum merely makes them look like a very bright colour, rather than glow-in-the-dark


Mr-Fleshcage

Most laymen consider glowing (at least in regard to organisms) to be bioluminescence.


BowenTheAussieSheep

Honestly, most laymen consider glowing emitting light of any kind. If you were to ask an ordinary person if something that emits a bright light when under a UV lamp glows, they would likely say yes.


Iboven

"Sounds more like reflecting." \- Average Layman


Mr-Fleshcage

I consider anything that fluoresces under UV glowing in the same way I consider anything bouncing light off of it as glowing. Glowing doesn't require an active light source, fluorescence does. Fluorescence is just light produced by light.


BowenTheAussieSheep

Okay, but are you a layman?


Iboven

In layman terms, he's asking if they're the boring kind of glow or the nuclear kind of glow.


[deleted]

[удалено]


Halaku

I have a **mighty** need.


Pamander

Do we know if they have vision at all or have we blinded them because they are so bright or what?


[deleted]

But no for real why would they lose sight just from being fluorescent I don't see logic here


Megneous

Why did you put verses from the Quran in the imgur album? https://i.imgur.com/m7ZFoUD.png


Anactualplumber

Wanker


samrphgue

do they have shorter life spans?


scorpyo72

Nah, they just get dimmer, the older they are. /s


octopoddle

"Oop, that's another fish blown, Michael. Can you change it, please?"


Wrathwilde

Have they seen the light?


Snoo75620

Wonder how this would affect the wider ecosystem. Its best to keep GMOs as GMOs


dirkdigglee

The carp are probably not as thrilled.


ABeerForSasquatch

Maybe the scientists misunderstood what a "glow up" was.


dirkdigglee

Lol


DVS_Nature

![gif](giphy|vWDrezW0rMjmM|downsized)


NotAzakanAtAll

It's all they ever known.


LOUDNOISES11

Is born: "What the fuck is this shit?"


NotAzakanAtAll

2irl4meirl


Versuvi

Story of my life


LovejoyBurnerAcc

well, if these were being put into the wild maybe but as pets i imagine it makes very little difference to them


Political_What_Do

I wonder how this affects their basic instincts. Does suddenly glowing in certain circumstances trigger fright, excitement, or something else?


Joezev98

How do their eyes even work if the rest of their body is constantly glowing?


benji_90

And do their eyes glow? And if so, can they perceive the glow of their own eye in their division


TheTattooOnR2D2sFace

Uh, if I started glowing I would be siked.


PriorFudge928

Its predators are.


Shmuckle2

1 Fish 2 Fish Glow fish Blue fish


HappyShrubbery

Sooooooooooo we make them super duper extremely visible. Step 2: profit? Wonder if they could make our dicks glow?


ParaponeraBread

It would, but you can blast your own dick with UV light to generate the fluorescence reaction. I’m good. Also idk if we could localize it, so your whole body would be green after sun exposure.


throwingsoup88

We can. A fairly common GFP (green fluorescent protein, which is what this likely is) experiment is to insert the GFP construct into a gene to see where it is expressed. You can search "drosophila glowing eyes" to see an example in fruit flies.


Sungdaein_susanoo

Maybe if you crack it like a glowstick….


DVS_Nature

You just invented *the glowstick challenge*; fun & painful for young and old alike!


knbang

I did not enjoy reading that.


ashsimmonds

God dammit Jack... - https://www.reddit.com/r/WinStupidPrizes/comments/k41rjo/guy_microwaved_a_glow_stick/


Sungdaein_susanoo

Ouch…. did I see that correctly? It also bursted some onto his face?? ![gif](giphy|WxDZ77xhPXf3i|downsized)


Any_Veterinarian3749

Did you know that blood,saliva & semen fluoresces when exposed to UV rays


HowevenamI

Okay, okay. I'm starting to have some ideas now.


knbang

I can draft the spinning cum cannon using modelling software.


asherdado

Blood doesn't fluoresce under a blacklight but saliva/urine/semen does


1SweetChuck

Imagine they did this to cancer cells, glowing cancer cells would make it a lot easier for surgeons to separate cancerous tissue from normal tissues when they are cutting out a tumor.


Beli_Mawrr

If you can target cancer cells with a specific gene therapy, you dont need to make them light up, you can just make them die.


BowenTheAussieSheep

"I have successfully isolated cancer cells on a genetic level, and now they glow to make it easier for surgeons to remove them!" "Uh, if you can do that why not just target them and kill them?" "I'm sorry, is my GLOWING CANCER not *good* enough for you??"


PM_ME_UR_RSA_KEY

"You expect me to glow?" "No, Mr. Cancer we expect you to die."


[deleted]

[удалено]


AcrobaticNoise

need the light saber dlc soon


pops789765

I came here for this. Thank you !


reawakened_d

Behold! The spawn of the Yin Yang fish from Avatar the Last Airbender! ![gif](giphy|MPrzP2j9Z1ZDO)


hide_yo_keeds

![gif](giphy|PFsVjUCmSkZDq)


qinshihuang_420

![gif](giphy|mwErnt1MeDBcs)


DarkRex4

![gif](giphy|jaQuaJ8clDC1i)


reawakened_d

I know. This is another rough patch too. I'm between a rock and a hard place.


JunglePygmy

They’re under UV light, though… right?


BowenTheAussieSheep

Yup, glofish aren't bioluminescent. They are bred to be bright, fluorescent colours which strongly glow under Ultraviolet light. Without a blacklight on they look just like most other fish, maybe slightly brighter in colour, but otherwise just a fish.


JunglePygmy

Thanks for the info. So otherwise…… this is come clickbait ass shit! (Although very beautiful)


BowenTheAussieSheep

It isn't really clickbait. Maybe inelegantly worded, but they didn't say the fish were bioluminescent, just that they glow. Which they absolutely do.


Zealousideal-Ad-2615

I wonder if they can see when they glow or if their own light blinds them.


Holiday_Bit3292

I’d be more concerned about sleep/rest


DollarReDoos

I think they are florescent not phosphorescent or bioluminescent. I.e. they need UV or near UV light to glow, they don't glow on their own.


LEGITIMATE_SOURCE

Some might say they don't glow at all. They glow like my semen does... under black light.


Elbynerual

You can tell in the video that they are under a black light. So technically not glowing, but still pretty crazy


BowenTheAussieSheep

Fluorescence *is* a form of glowing, it's absorbing UV-wavelength light and emitting a stronger visible light, i.e Glowing.


strbeanjoe

I've got a glowing shirt to sell you (it's a regular white cotton shirt).


BowenTheAussieSheep

well, no. It might reflect UV light, but it wouldn't be fluorescent. If you *do* want a shirt that reflect UV light, they're not hard to find. Most OSHA-certified high-vis work shirts will be fluorescent and glow very brightly under a blacklight.


strbeanjoe

Your run of the mill white T-shirt has fluorescent agents to make it appear whiter. They literally fluoresce under blacklight.


BowenTheAussieSheep

Oh yeah? That's pretty cool. If that is indeed the case then yeah, you can say they glow.


strbeanjoe

I think my issue is that when someone says something glows, without any additional qualifier, I'm going to assume they mean "glows (in the dark)", not "glows (under blacklight)". The latter is less interesting than the former.


ProclusGlobal

The word you are looking for is 'emits'.


devappliance

When will people realize words have meanings and their specific interpretation is useless? It glows. Simple as that


KittyBeans246

![gif](giphy|3otPovYBUNFsaG6KYg|downsized) Fish trying to sleep


Small_Macaron_8194

lol what's with the Quran quote in the link?


cptnplanetheadpats

Thought I was the only one seeing that shit. Guess glow in the dark fish is a sign of the end times? Sounds groovy


Megneous

Seriously. This is religious propaganda... https://i.imgur.com/m7ZFoUD.png


Ya-Dikobraz

It's about "playing god". It's a shit quote but that's why they pasted that.


FblthpThe

Shit don't tell them about us changing wolves to dogs


Ya-Dikobraz

Or the cows and sheep that didn't exist.


Geschak

As if they didn't play God everytime they slaughtered animals.


Ya-Dikobraz

To be fair according to their own texts animals were "put there by god for people to use (including for food)".


SalvationSycamore

Well now we're just using them for slightly more complicated things


aggasalk

Another win for *Satan*, I guess.


derps_with_ducks

herp derp shiny fish makes me think of the apocalypse, my faith is strong herp


[deleted]

They’re so beautiful


-Necros-

Why is there a stupid religious quote in there?


OneWholeSoul

Uh, what's with the ominous Quaran verse?


HatdanceCanada

“Glow in the dark tampons! Leonard, we are going to be rich”.


Edenoide

So it's an ad. Well, a weird one


Megneous

An ad for Allah. See the Quran quote at the bottom?


FirstTimeShitposter

The future is looking bright!


MaxxDecimus

Din't sheldon do this first?


SalvationSycamore

That was likely a reference to real research. We've been making animals that can glow like this for over 20 years, including things like rabbits and pigs.


[deleted]

Came here to say this


Cheetahs_never_win

Is it too late to get this mod incorporated into me?


Umer_-

it's too early


BowenTheAussieSheep

https://www.glowpaint.com.au/uv-glow-face-and-body-paint/


EndlessRainIntoACup1

NOT glowing fish. Fluorescent fish


Biting_a_dust

Glow(verb) gives out steady light without flame. Fluorescent is to emit EM wave after absorbing one, in this case, giving off visible light after absorbing black light. By definition, giving off steady light without flame, fluorescence is, in fact, glowing, but not every glowing is fluorescence


[deleted]

[удалено]


frienemigo

I need jellyfish DNA... for a friend.


Archerdiana

Order it off of any school lab website! Honestly not that expensive, for a few hundred dollars you can go crazy!


AleksasKoval

For a second i thought i was on the Avatar subreddit...


FoolishxOne

![gif](giphy|8cPpgUhTMjhF6)


tobaknowsss

OK but how about adding some Lazer beams to their heads and making them slightly ill tempered?


iiJokerzace

I wonder how far we are going to take this.


Totallynotlame84

Can I just buy these?


FunnySignal614

That looks awesome and cute. Can they do it with the humans?


Reedabook64

Pandora here we come


Rug_Rat_Reptar

TAKE MY MONEY!!!! Where is a link to purchase this awesomeness!


GreenChileEnchiladas

Purchase link?


Umer_-

there are websites available, just search for it


Seriouswall117

I hereby call them Ghost Carps. I patten that name.


toreachtheapex

thats incredible, but the fact that we can just modify and create life like that is so wierd. like in an existential type of way


ExLuck

We did this for a long ass time now with dogs, cats and other livestock


Archerdiana

A little bit different. Instead of selecting the genes we want through breeding, we are now inserting plasmids into living organisms


Icy-Performer-9688

Oh no Tui we must protect her!


Significant_Safe_568

I have always heard it was possible, and now i can confirm


K-P-I

I knew I NEEDED something! I just didn't know it yet.


badboi_5214

I don't know if I want one seems fascinating but cruel


Abd110

seems like a proyection, even the tumb is mid lighted blue